Comparative HPLC-MSn analysis of canine and human meibomian lipidomes: many similarities, a few differences
نویسندگان
چکیده
The aim of this study was to evaluate the lipidome of meibomian gland secretions in canines (cMGS) - a common pet and laboratory animal - and to compare it with that of human MGS (hMGS), to determine whether canines could be used as a valid experimental animal model in studies of the biochemistry and physiology of the human ocular surface in general, and of the Meibomian glands in particular. The MGS of both species were evaluated using HPLC in combination with atmospheric pressure chemical ionization ion trap mass spectrometry. The main lipid classes found in cMGS were very long chain cholesteryl esters, wax esters, (O-acyl)-omega-hydroxy fatty acids (OAHFA), and cholesteryl esters of OAHFA. The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed.
منابع مشابه
A comparative study of religious and non-religious lifestyle : Emphasizing health behavior and cardiovascular diseases
Abstract Goal: The purpose of this study was to review the differences between religious and non-religious lifestyle in health behaviors with a particular emphasis on cardiovascular health. Method: The method of this study was the analytical method. Results: The review of researches suggested that the religious and non-religious health lifestyle have some differences and similarities and the...
متن کاملComparative Analysis of Two Seismic Response Analysis Programs in the Actual Soft Field
SHAKE2000 and DEEPSOIL are the two most important programs to calculate the response analysis of soil layer. In order to analyze the similarities and differences between them, and to guide the improvement of the method and program of seismic response analysis of soil layer, 25 KiK net seismic records from 9 stations were selected as the research objects in this paper, from the aspects of surfac...
متن کاملThe comparative study of Girih tiles ornamentation in the historical villages of Masouleh and Abyaneh
From the past to the present, the historical villages of Masouleh and Abyaneh have had many similarities in the fields of decoration and architecture despite the climatic, geographical and ancient differences, and the existence of such commonalities has always been of interest to many researchers. Considering that the use of decorations such as gereh-chini is being forgotten in contemporary tim...
متن کاملA Comparative Study on Lachak and Toranj Design Between Joshaghan of Kashan and Saman of Bakhtiari Region
Lachak Toranj is one of the most used designs in Persian carpets. There are many different Lachak Toranj styles one of which is used to weave carpets in Joshaghan region located in Esfahan province. This style is in some ways similar to some carpets woven using the Lachak Toranj style of Saman region located in Chaharmahal-Va-Bakhtiari province. These similarities despite the long distance b...
متن کاملIdentification of chemical constituents in the extract and rat serum from Ziziphus jujuba Mill. by HPLC-PDA-ESI-MSn
Chinese jujube (Ziziphus jujuba Mill.) has long been widely used for human consumption and medicinal purposes in China. It has been reported to possess several vital biological activities. However, the systematic study on the chemical constituents absorbed into plasma and their metabolites is still insufficient. A high-performance liquid chromatography-photodiode array detector-electrospray ion...
متن کامل